Temporal Changes in Heat Shock Protein Levels (HSP70 and HSP90) at Rest and During the First Hour After a High-Intensity Resistance Exercise Session in Weightlifters
DOI:
https://doi.org/10.37359/JOPE.V38(2)2026.2456الكلمات المفتاحية:
Heat shock proteins، HSP70، HSP90 resistance exercise، acute physiological response، weightliftingالملخص
This study was about how heat shock proteins, HSP70 and HSP90 change in the body after a really tough workout and over the next hour in top weightlifters. Heat shock proteins like HSP70 and HSP90 help us figure out how the body deals with stress from exercise.
We worked with 12 weightlifters from Amanat Baghdad Sports Club. They did a resistance workout that included the snatch and clean & jerk exercises at 85–90% of their maximum lift.
We took blood from these weightlifters at four times: before they started the workout right after they finished 30 minutes later and 60 minutes later.
We used a test called ELISA to check the levels of HSP70 and HSP90 in their blood.
The results showed that the levels of HSP70 went up a lot right after the weightlifters finished exercising and stayed high for a while. Heat shock proteins like HSP70 were still high in their blood, after some time. They went from 1.92 ± 0.38 ng/mL before exercise to 2.64 ± 0.45 ng/mL after and peaked at 30 minutes (2.98 ± 0.50 ng/mL) before going down a bit at 60 minutes. HSP90 levels did something. They went up from 2.73 ± 0.47 ng/mL before exercise to 3.35 ± 0.52 ng/mL after peaked at 30 minutes (3.68 ± 0.56 ng/mL) and then went down a bit.
These findings tell us that a single tough workout makes heat shock proteins, like heat shock proteins go up fast in weightlifters.
This increase in heat shock proteins happens after you do your exercise and it peaks within thirty minutes. Then it goes down over the next sixty minutes.
This shows that your body is turning on things to help with stress from exercise like the stress from a workout.
The results also suggest that HSP70 and HSP90 could be things to look at to track how stress your body is, under and to manage your workout loads in people who do strength training like strength athletes who do strength training.
المراجع
Akbar, M., Figueiredo, V. C., Bloomer, R., & Ferreira, S. E. (2024). Blood biomarkers in response to high-intensity exercise: Clinical and performance implications. Sports Medicine, 54(2), 307–322.
Ali, H., & Yasser, O. K. (2018). Antioxidant supply and its effect on concentration of collagen in national league weightlifters’ blood. Journal of Physical Education, 30(2), 379–394. https://doi.org/10.37359/JOPE.V30(2)2018.372
Ali, H., & Yasser, O. K. (2018). Effect of antioxidants on the achievement level of National Weightlifting League. Journal of Physical Education, 30(2), 395–407. https://doi.org/10.37359/JOPE.V30(2)2018.372
Babraj, J., Fallowfield, J. L., & Maughan, R. J. (2021). Hematological and biochemical responses to resistance exercise: A systematic review. Journal of Strength and Conditioning Research, 35(10), 2839–2848.
Febbraio, M. A., Ott, P., Nielsen, H. B., & Krustrup, P. (2018). Exercise-induced heat shock protein response in skeletal muscle. Physiological Reviews, 98(3), 1035–1073.
Febbraio, M. A., Ott, P., Nielsen, H. B., & Krustrup, P. (2018). Exercise-induced heat shock protein response in skeletal muscle. Physiological Reviews, 98(3), 1035–1073.
Giacomin, C., Sleivert, G., & Abouassi, H. (2022). Acute responses of heat shock proteins to high intensity resistance exercise in trained men. Journal of Applied Physiology, 133(1), 45–55.
González-Haro, C., et al. (2022). Acute heat shock protein responses to high-intensity resistance exercise. European Journal of Sport Science, 22(8), 1194–1203.
González-Haro, C., et al. (2022). Acute heat shock protein responses to high-intensity resistance exercise. European Journal of Sport Science, 22(8), 1194–1203.
Ho, R., Chen, Y., & Wong, A. (2024). Temporal dynamics of HSP70 and HSP90 following exhaustive strength training: A systematic review. European Journal of Sport Science, 24(4), 502–514.
jasem, Z. K., & Yasser, O. K. (2024). Obesity and its relationship with psychological inactivity in female students of the college of physical education and sports sciences. Wisdom Journal For Studies & Research, 4(04), 1411–1419. https://doi.org/10.55165/wjfsar.v4i04.279
Liu, X., Zhang, Y., & Li, J. (2024). Immediate alterations in HSP70 and HSP90 expression after high resistance exercise: Implications for muscle adaptation. Journal of Sports Sciences, 42(2), 159–168.
Liu, Y., Zhang, H., & Wang, L. (2023). Exercise-induced regulation of heat shock proteins in skeletal muscle. International Journal of Molecular Sciences, 24(5), 4562.
Liu, Y., Zhang, H., & Wang, L. (2023). Exercise-induced regulation of heat shock proteins in skeletal muscle. International Journal of Molecular Sciences, 24(5), 4562.
Liu, Y., Zhang, H., & Wang, L. (2023). Exercise-induced regulation of heat shock proteins in skeletal muscle. International Journal of Molecular Sciences, 24(5), 4562.
Mizuno, K., Hamada, T., & Akimoto, T. (2020). Mechanisms of heat shock protein regulation in skeletal muscle following exercise. Exercise and Sport Sciences Reviews, 48(3), 120–129.
Morton, J. P., Murphy, K. T., McKellar, S. R., & Drust, B. (2021). Interactions between exercise and heat shock proteins in skeletal muscle adaptation. Sports Medicine, 51(4), 713–728.
Morton, J. P., Murphy, K. T., McKellar, S. R., & Drust, B. (2021). Interactions between exercise and heat shock proteins in skeletal muscle adaptation. Sports Medicine, 51(4), 713–728.
Morton, J. P., Murphy, K. T., McKellar, S. R., & Drust, B. (2021). Interactions between exercise and heat shock proteins in skeletal muscle adaptation. Sports Medicine, 51(4), 713–728.
O. K., Saeed, T. A., & Fenjan, F. H. (2020). The effect of high training stress on each of concentration (blood urine) on Iraqi national team weightlifters. International Journal of Psychosocial Rehabilitation, 24(4), 9716–9720. https://doi.org/10.61841/35d9yv92
Peake, J. M., Neubauer, O., Della Gatta, P., & Nosaka, K. (2015). Muscle damage and inflammation during recovery from exercise. Journal of Applied Physiology, 122(3), 559–570.
Peake, J. M., Neubauer, O., Della Gatta, P., & Nosaka, K. (2015). Muscle damage and inflammation during recovery from exercise. Journal of Applied Physiology, 122(3), 559–570.
Peake, J. M., Neubauer, O., Della Gatta, P., & Nosaka, K. (2015). Muscle damage and inflammation during recovery from exercise. Journal of Applied Physiology, 122(3), 559–570.
Rodrigues, B., Caperuto, E. C., & de Souza Genaro, P. M. (2021). Heat shock proteins responses in skeletal muscle after strength training: A meta analytic review. Strength and Conditioning Journal, 43(6), 88–98.
Saeed, T. A., Yasser, O. K., & Fenjan, F. H. (2020).The effect of various aerobic exercises on the endurance and some physiological variables among fitness training practitioners of at (30-35) years old. International Journal of Psychosocial Rehabilitation, 24(3), 6083–6086. https://doi.org/10.61841/9pw2k090
Scott, S. J., Grindstaff, P. D., & Fragala, M. S. (2022). Cellular stress responses to resistance training: A narrative review of molecular and physiological adaptations. Sports Medicine, 52(8), 1701–1722.
Taipale, M., Jarosz, D. F., & Lindquist, S. (2020). HSP90 at the hub of protein homeostasis. Nature Reviews Molecular Cell Biology, 21(9), 515–528.
Taipale, M., Jarosz, D. F., & Lindquist, S. (2020). HSP90 at the hub of protein homeostasis. Nature Reviews Molecular Cell Biology, 21(9), 515–528.
Taipale, M., Jarosz, D. F., & Lindquist, S. (2020). HSP90 at the hub of protein homeostasis. Nature Reviews Molecular Cell Biology, 21(9), 515–528.
Vissing, K., & Schjerling, P. (2020). Heat shock proteins in human skeletal muscle: Regulation and function. Physiology Reviews, 100(2), 627–700.
Yasir, O. K. (2014). Exercises with different ranges of motion with significance of electrical activity for muscle in strength with speed of lower limbs for weightlifters of physical strength. Journal of Physical Education, 26(4). Retrieved from https://jcope.uobaghdad.edu.iq
Yasir, O. K. (2025). The percentage of cotinine in the body and its relationship with the level of achievement of the snatch in weightlifting. Annals of Applied Sport Science, 13(3), Article e1514. https://doi.org/10.61882/aassjournal.1514
Yasir, O. K., Ali, M. H., & Alhusseini, A. M. J. (2025). The effect of a nutritional program on the electromyographic activity (EMG) of selected muscles in weightlifters during the snatch lift. Retos, 70, Article 117105. https://doi.org/10.47197/retos.v70.117105
Yasir, O. K., Naji, Z. F., & Mukheef, S. M. (2025). The relationship between essential mineral elements and snatch lift performance in weightlifters: An analytical study. Annals of Applied Sport Science, 13(4), Article e1523. https://doi.org/10.61882/aassjournal.1523
Yasser, O. K. (2021). The Effect Of A Nutritional Program On Muscle Strength And Some Physical Measurements Of The Quadruple Of Kadhimiya Sports Club For Weightlifting. Turkish Journal of Physiotherapy and Rehabilitation.
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